Summary
For excluding deep-vein thrombosis (DVT), a negative D-dimer and low clinical probability
are used to rule out DVT. Circulating microRNAs (miRNAs) are stably present in the
plasma, serum and other body fluids. Their diagnostic function has been investigated
in many diseases but not in DVT. The aims of present study were to assess the diagnostic
ability of plasma miRNAs in DVT and to examine their correlation with known markers
of hypercoagulability, such as D-dimer and APC-PCI complex. Plasma samples were obtained
from 238 patients (aged 16–95 years) with suspected DVT included in a prospective
multicentre management study (SCORE). We first performed miRNA screening of plasma
samples from three plasma pools containing plasma from 12 patients with DVT and three
plasma pools containing plasma from 12 patients without DVT using a microRNA Ready-to-use
PCR Panel comprising 742 miRNA primer sets. Thirteen miRNAs that differentially expressed
were further investigated by quantitative real-time (qRT)-PCR in the entire cohort.
The plasma level of miR-424–5p (p=0.01) were significantly higher, whereas the levels
of miR-136–5p (p=0.03) were significantly lower in DVT patients compared to patients
without DVT. Receiver-operating characteristic curve analysis showed the area under
the curve (AUC) values of 0.63 for miR-424–5p and 0.60 for miR-136–5p. The plasma
level of miR-424–5p was associated with both D-dimer and APC-PCI complex levels (p<0.0001
and p=0.001, respectively). In conclusions, these findings indicate that certain miRNAs
are associated with DVT and markers of hypercoagulability, though their diagnostic
abilities are probably too low.
Supplementary Material to this article is available online at www.thrombosis-online.com.
Keywords
Deep venous thrombosis - microRNA - diagnosis - D-dimer - APC-PCI complex
